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The development of a treatment model for lymph node metastasis in nude mice transplanted with rat fibrosarcoma clone
Y Soejima1, W M Shen, K Minato
1Pharmacology Division, National Cancer Center Research Institute, Tokyo, Japan.
Abstract:
We have tried to establish a model for lymph node metastasis in nude mice transplanted with clone A of methylcholanthrene-induced rat fibrosarcoma. Tumor cells transplanted either subcutaneously or intrafootpad metastasized exclusively to the lymph nodes in nude mice treated with antiasialo GM1 antiserum. In the experiments of intrafootpad transplantation, all the mice died of disseminated lymph node metastasis if the leg was not resected, or was resected more than 3 weeks after the transplantation of clone A. Some mice whose legs were amputated 1 or 2 weeks after transplantation could be cured by amputation alone. The maximum effective dose of in vitro active drug could improve the survival in mice, and 3/5, 4/5, and 3/5 mice treated with MMC, ACNU and CDDP respectively were cured even if their legs were amputated 3 weeks after transplantation. This tumor-bearing animal system was considered to provide a valuable experimental model for lymph node metastasis.
Insights
Researchers developed a nude mouse model for studying lymph node metastasis using methylcholanthrene-induced fibrosarcoma. This model effectively demonstrated metastasis and tested anti-cancer drugs, showing potential for future research.
Area of Science:
- Oncology
- Immunology
- Animal Models
Background:
- Lymph node metastasis is a critical factor in cancer progression and patient survival.
- Developing reliable animal models is essential for understanding metastasis and evaluating therapeutic strategies.
Purpose of the Study:
- To establish a reproducible animal model for studying lymph node metastasis.
- To investigate the efficacy of different treatment timings and chemotherapeutic agents in managing metastasis.
Main Methods:
- Utilized clone A of methylcholanthrene-induced rat fibrosarcoma in a nude mouse model.
- Administered antiasialo GM1 antiserum to facilitate lymph node metastasis.
- Performed surgical resection of affected limbs at various time points post-transplantation.
- Evaluated the impact of chemotherapeutic drugs (MMC, ACNU, CDDP) on survival rates.
Main Results:
- Tumor cells consistently metastasized to lymph nodes in treated nude mice.
- Limb resection within 1-2 weeks post-transplantation led to cure in some mice.
- Chemotherapy improved survival, with significant cure rates even when limbs were amputated 3 weeks after transplantation.
Conclusions:
- The established nude mouse model is valuable for experimental lymph node metastasis research.
- Early detection and intervention, including surgical resection and chemotherapy, are crucial for managing metastasis.
- The model supports the evaluation of chemotherapeutic efficacy in preventing or treating lymph node metastasis.