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Related Experiment Video

Updated: Jun 23, 2026

Short-Duration Hypothermia Induction in Rats using Models for Studies examining Clinical Relevance and Mechanisms
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Published on: March 3, 2021

Cold ischemia does not interfere with tolerance induction.

Anja Reutzel-Selke1, Jan Hartmann, Paul Brandenburg

  • 1Department of Surgery, Charité, Campus Virchow Clinic, Universitätsmedizin Berlin, Germany.

Transplantation
|April 23, 2009
PubMed
Summary
This summary is machine-generated.

Prolonged cold ischemia does not hinder tolerance induction in kidney transplants. This extended ischemia may even enhance tolerance by reducing immune responses in adoptive cell transfer recipients.

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Area of Science:

  • Transplantation immunology
  • Organ transplantation
  • Immune tolerance induction

Background:

  • Ischemia/reperfusion injury activates innate immunity, potentially hindering immune tolerance.
  • The impact of prolonged cold ischemia on successful tolerance induction remains unclear.

Purpose of the Study:

  • To investigate whether prolonged cold ischemia interferes with successful tolerance induction in kidney transplantation.
  • To assess the effect of prolonged cold ischemia on immune responses following tolerance induction.

Main Methods:

  • Kidney grafts from Dark Agouti donors were transplanted into Lewis rats after short (20 min) or prolonged (6 hr) cold ischemia.
  • Tolerance was induced using a nondepleting anti-CD4 monoclonal antibody (RIB 5/2).
  • Adoptive transfer experiments were conducted to evaluate immune responses.

Main Results:

  • All RIB 5/2-treated recipients survived, regardless of cold ischemia time.
  • Graft function and morphology were comparable between short and prolonged ischemia groups.
  • Prolonged cold ischemia led to reduced donor-derived cells and an attenuated immune response post-adoptive transfer.

Conclusions:

  • Extended cold ischemia does not impede tolerance induction by RIB 5/2.
  • Prolonged cold ischemia may act as a 'tolerizing conditioning,' reducing immune responses in adoptive cell transfer recipients.