Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 23, 2026

A High Output Method to Isolate Cerebral Pericytes from Mouse
06:49

A High Output Method to Isolate Cerebral Pericytes from Mouse

Published on: January 14, 2020

Pericytes display increased CCN2 expression upon culturing.

Xu Shiwen1, Vineeth Rajkumar, Christopher P Denton

  • 1Centre for Rheumatology, University College London (Royal Free Campus), Rowland Hill Street, London, UK, NW3 2PF.

Journal of Cell Communication and Signaling
|April 23, 2009
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Crosstalk of the Hippo/YAP pathway in the progression of oral potentially malignant disorders to oral squamous cell carcinoma: a systematic review.

Frontiers in oral health·2026
Same author

SYISL promotes pulmonary epithelial-mesenchymal transition and fibrosis through DSP-Hippo/YAP pathway.

Cellular and molecular life sciences : CMLS·2026
Same author

A Pan-Cancer Transcriptomic Signature for Conserved Molecular Programs Underlying Premalignant-Malignant Progression Across Common Carcinomas.

Dentistry journal·2026
Same author

CCN1: a SASPy protein that plays multifaceted roles in fibrogenesis.

Matrix biology : journal of the International Society for Matrix Biology·2025
Same author

The Areca Nut and Oral Submucosal Fibrosis: A Narrative Review.

Dentistry journal·2025
Same author

George R. Martin: Pioneering matrix biologist.

Matrix biology : journal of the International Society for Matrix Biology·2025
Same journal

Ceramide-mediated mitochondrial dysfunction in nonobese nonalcoholic fatty liver disease: A regulatory role for serine palmitoyltransferase subunit 2.

Journal of cell communication and signaling·2026
Same journal

Correction to Regulation of phosphatase and tensin homolog by complement component 5a (C5a) and its receptor (C5aR 1) in lupus nephritis: A novel therapeutic target.

Journal of cell communication and signaling·2026
Same journal

Plasma exosomal miR-339-3p promotes myocardial remodeling in chronic heart failure by regulating USP25-mediated DDX58 deubiquitination.

Journal of cell communication and signaling·2026
Same journal

A working model for CCN3 C-terminal domain-mediated transcriptional modulation of the plasminogen activation system.

Journal of cell communication and signaling·2026
Same journal

Inflammatory cytokine IL-6 regulates ADAMTS14 expression through MAPK and PI3K signaling in colorectal cancer.

Journal of cell communication and signaling·2026
Same journal

Gemcitabine activates the Hippo signaling pathway and suppresses tumor growth by stabilizing large tumor suppressor kinase 2 through the hypoxia-inducible factor 1-alpha/ubiquitin protein ligase E3 component N-recognin 5 axis.

Journal of cell communication and signaling·2026
See all related articles

Microvascular pericytes can transform into fibroblast-like cells in vitro. This suggests pericytes may contribute to fibroblast populations during wound healing and tissue repair.

Area of Science:

  • Cell Biology
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Microvascular pericytes are known to support tissue repair by differentiating into alpha-smooth muscle actin (alpha-SMA)-expressing myofibroblasts.
  • The potential contribution of pericytes to the fibroblast population after tissue repair remains largely unexplored.

Purpose of the Study:

  • To investigate whether pericytes isolated from human placenta can acquire a fibroblast-like phenotype in vitro.
  • To understand the cellular plasticity of pericytes in the context of wound healing.

Main Methods:

  • Human placental pericytes were cultured in vitro for up to 11 passages.
  • Affymetrix mRNA expression profiling was performed on early (passage 2) and late (passage 11) passage pericytes.
  • Protein expression of key fibroblast markers was assessed.

More Related Videos

Culture of Brain Capillary Pericytes for Cytosolic Calcium Measurements and Calcium Imaging Studies
09:33

Culture of Brain Capillary Pericytes for Cytosolic Calcium Measurements and Calcium Imaging Studies

Published on: May 27, 2020

Related Experiment Videos

Last Updated: Jun 23, 2026

A High Output Method to Isolate Cerebral Pericytes from Mouse
06:49

A High Output Method to Isolate Cerebral Pericytes from Mouse

Published on: January 14, 2020

Culture of Brain Capillary Pericytes for Cytosolic Calcium Measurements and Calcium Imaging Studies
09:33

Culture of Brain Capillary Pericytes for Cytosolic Calcium Measurements and Calcium Imaging Studies

Published on: May 27, 2020

Main Results:

  • Passaging pericytes in culture induced the expression of type I collagen, thrombospondin, and fibronectin mRNAs.
  • This shift in gene expression was accompanied by increased connective tissue growth factor (CTGF/CCN2) and type I collagen protein levels.
  • The fibroblast marker ASO2 was also induced, confirming the phenotypic change.

Conclusions:

  • Human placental pericytes possess the intrinsic capacity to differentiate into fibroblast-like cells under specific culture conditions.
  • These findings suggest that pericytes may serve as a source of fibroblasts in the cellular milieu of a healed wound, contributing to matrix remodeling.