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[Studies on application of ultra-filtration to purifying polysaccharides from Rhubarb]
Yu Sun1, Yongchuan Zhou, Dexu Chen
1Modern Engineering Center for Chinese Traditional Medicine, East China University of Science and Technology, Shanghai 200237, China.
Objective:
To purify Rhubarb polysaccharides via micro-filtration and ultra-filtration.
Method:
The technology adopted micro-filtration and tubular membrane with different cut-off molecular weights.
Result:
Experiment results showed that the optimum operating conditions were that the ultra-filtration time controlled around 80 min, the temperature was set the 35-40 degrees C, the operating pressure was 0.08-0.12 MPa, the pH was 6-8, and a sample that of polysaccharide was 0.5 times of original concentration. The recovery ratio of the polysaccharides was 53.7%, the concentration could be condensed to 2.73 times compared with the sample liquid.
Conclusion:
The technology was simple and feasible, it can be applied to purify Rhubarb polysaccharides.
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