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Labeling DNA Probes

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Updated: Jun 23, 2026

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

Published on: June 3, 2018

[Research on DNA fluorescence capillary biosensor marked by Goldview].

Yan-Jun Wang¹, Yong-Sheng Li, Quan-Yu Yang

¹West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.

Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu

|April 24, 2009

Summary

A novel DNA biosensor using fluorescence capillary analysis (FCA) offers a simple, cost-effective method for DNA detection. This DNA fluorescence capillary biosensor (DNA-FCB) minimizes sample volume and environmental impact.

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Last Updated: Jun 23, 2026

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen

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Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Published on: June 1, 2011

Area of Science:

Analytical Chemistry
Biotechnology
Biosensing Technology

Background:

DNA detection is crucial in various fields, including diagnostics and environmental monitoring.
Existing methods can be complex, require large sample volumes, or be costly.
Development of sensitive, efficient, and user-friendly DNA detection systems is ongoing.

Purpose of the Study:

To develop and characterize a novel Goldview-marked DNA fluorescence capillary biosensor (DNA-FCB).
To evaluate the performance of the DNA-FCB for qualifying and quantifying target DNA.
To highlight the advantages of the developed biosensor in terms of simplicity, sample volume, and cost.

Main Methods:

Immobilization of DNA probes (20-mer-ssDNA) onto the inner wall of a capillary using poly-l-lysine.
Hybridization of immobilized probes with complementary target DNA.
Staining with Goldview dye and detection of fluorescence intensity using a spectrofluorometer (F-4500).
Utilizing fluorescence capillary analysis (FCA) for detection.

Main Results:

The DNA-FCB demonstrated good linearity between target DNA concentration and fluorescence intensity (0.4-4 µmol/L).
The correlation coefficient (r) was high (0.9989), indicating excellent linearity.
The relative standard deviation (RSD) was below 3.5%, demonstrating good reproducibility.
A low detection limit of 0.39 µmol/L for target DNA was achieved.

Conclusions:

The developed DNA fluorescence capillary biosensor (DNA-FCB) is effective for both qualitative and quantitative DNA detection.
The biosensor offers significant advantages, including ease of use, minimal sample and reagent requirements, reusability, and low cost.
This method presents a more environmentally friendly approach to DNA analysis, reducing pollution.