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UV–Vis Spectrometers

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...

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Related Experiment Video

Updated: Jun 23, 2026

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

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Published on: January 9, 2017

[Decomposing total suspended particle absorption based on the spectral correlation relationship].

Gui-Fen Wang¹, Wen-Xi Cao, Ding-Tian Yang

¹LED Laboratory, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China. guifenwang@scsio.ac.cn

Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu

|April 24, 2009

Summary

A new model accurately separates phytoplankton and nonalgal particle absorption, crucial for ocean color remote sensing. This method improves phytoplankton absorption retrieval from in situ and satellite data.

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Last Updated: Jun 23, 2026

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

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Published on: June 5, 2019

Area of Science:

Oceanography
Remote Sensing
Biogeochemistry

Context:

Particulate absorption is key to ocean color.
Phytoplankton and nonalgal particles influence total absorption.
Accurate separation is needed for biogeochemical studies.

Purpose:

Develop a model to partition total particulate absorption into phytoplankton and nonalgal components.
Establish spectral relationships for phytoplankton and nonalgal particle absorption.
Validate the model using independent measurements.

Summary:

A novel model decomposes total particulate absorption spectra using distinct phytoplankton and nonalgal particle relationships.
Phytoplankton absorption is modeled quadratically with aph(443), and nonalgal absorption exponentially.
The model shows high accuracy (r2 > 0.97) and consistency with in situ measurements.

Impact:

Provides an effective tool for local applications in oceanographic research.
Eliminates the need for pigment concentration data.
Aligns with ocean color satellite sensor bands for broader applicability.