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Updated: Jun 23, 2026

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Preparation of functional aptamer films using layer-by-layer self-assembly.

Yasir Sultan1, Ryan Walsh, Carlos Monreal

  • 1Department of Chemistry, Carleton University, Ottawa-Carleton Chemistry Institute, 1125 Colonel By Drive, Ottawa, Ontario, Canada K1S 5B6.

Biomacromolecules
|April 24, 2009
PubMed
Summary

Aptamers retain molecular recognition abilities when embedded in polyelectrolyte films. This study shows aptamer-based functional thin films maintain specificity and binding affinity for advanced applications.

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Area of Science:

  • Biomaterials Science
  • Molecular Recognition
  • Nanotechnology

Background:

  • Aptamer applications require understanding matrix effects on molecular recognition.
  • Sulforhodamine B aptamer is a model system for studying matrix incorporation.

Purpose of the Study:

  • To investigate if the sulforhodamine B aptamer retains binding ability within a multilayer polyelectrolyte film.
  • To compare aptamer-containing films with those containing DNA or random oligonucleotides.

Main Methods:

  • Layer-by-layer deposition of polyelectrolyte films.
  • Incorporation of aptamer, DNA, or random oligonucleotide as polyanions.
  • Characterization using UV-vis spectroscopy, quartz crystal microbalance, confocal microscopy, and TOF-SIMS.

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Main Results:

  • The aptamer retained its target recognition ability within the polyelectrolyte film.
  • No loss of specificity was observed.
  • A modest reduction in binding affinity was noted.

Conclusions:

  • Aptamer incorporation into polyelectrolyte films preserves molecular recognition.
  • This supports the development of aptamer-based functional thin films.
  • Opens opportunities for new aptamer-based material applications.