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Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
Published on: April 25, 2014
Utility and evaluation of new variable-number tandem-repeat systems for genotyping mycobacterial tuberculosis
Horng-Yunn Dou1, Jang-Jih Lu, Chih-Wei Lin
1Division of Clinical Research, National Health Research Institutes, Zhunan, Taiwan.
Abstract:
We compared mycobacterial interspersed repetitive unit (MIRU)-variable number tandem repeat (VNTR) typing to traditional spoligotyping for discriminating Mycobacterium tuberculosis (MTB) strains. Our 17-loci MIRU-VNTR typing method was found to be superior to spoligotyping for non-Beijing family strains. To extend the method we also established PCR-based rapid genotyping protocols for Beijing, East-African-Indian and U lineages.
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