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Updated: Jun 23, 2026
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In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
Published on: May 21, 2015
Are putative periodontal pathogens reliable diagnostic markers?
Birgit Riep1, Lilian Edesi-Neuss, Friderike Claessen
1Abteilung für Zahnerhaltung und Parodontologie, Institut für Mikrobiologie und Hygiene, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Journal of Clinical Microbiology
|April 24, 2009
Summary
This study found that while common periodontal pathogens were present in both diseased and resistant groups, Tannerella lecithinolyticum may indicate aggressive periodontitis, and Capnocytophaga ochracea suggests periodontal health.
Area of Science:
- Oral microbiology
- Periodontal disease research
- Infectious disease diagnostics
Background:
- Periodontitis is a common chronic inflammatory disease.
- Specific oral bacteria are linked to periodontitis and used as diagnostic markers.
- Understanding bacterial prevalence in different periodontitis forms is crucial.
Purpose of the Study:
- Compare oral bacterial species prevalence in generalized aggressive periodontitis (GAP), chronic periodontitis (CP), and periodontitis-resistant (PR) individuals.
- Identify potential bacterial diagnostic markers for specific periodontal conditions.
- Evaluate the association of bacterial species with probing depth versus disease group.
Main Methods:
- Analyzed 555 subgingival biofilm samples from 111 subjects (GAP, CP, PR) using PCR and dot blot hybridization.
- Targeted specific bacterial species including Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Tannerella lecithinolyticum, and Capnocytophaga ochracea.
- Correlated bacterial detection with probing depth and subject group.
Main Results:
- P. gingivalis, P. intermedia, and T. forsythia were prevalent in both periodontitis and PR groups.
- T. lecithinolyticum showed significant differences between GAP and CP, and between GAP and PR groups.
- C. ochracea was associated with PR subjects, irrespective of probing depth.
Conclusions:
- T. lecithinolyticum may serve as a diagnostic marker for generalized aggressive periodontitis.
- C. ochracea may indicate periodontal health.
- Current associations between specific bacteria and periodontal health/disease warrant further investigation.
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