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Related Experiment Video

Updated: Jun 23, 2026

Micro-drive Array for Chronic in vivo Recording: Tetrode Assembly
14:19

Micro-drive Array for Chronic in vivo Recording: Tetrode Assembly

Published on: April 22, 2009

Micro-drive array for chronic in vivo recording: tetrode assembly.

David P Nguyen1, Stuart P Layton, Gregory Hale

  • 1Department of Brain and Cognitive Science, Massachusetts Institute of Technology, USA. dpnguyen@mit.edu

Journal of Visualized Experiments : Jove
|April 24, 2009
PubMed
Summary

This protocol details fabricating and implanting tetrodes for chronic in-vivo neural recordings in rats. It enables precise, long-term monitoring of neural activity for studying brain function.

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Area of Science:

  • Neuroscience
  • Electrophysiology
  • Surgical Protocols

Background:

  • Tetrodes, bundles of four electrodes, are crucial for simultaneous multi-neuron recordings in vivo.
  • Action potential amplitude differences across tetrode channels enable single-unit isolation from multi-unit signals.
  • Precise stereotaxic placement and depth control are essential for studying coordinated neural activity.

Purpose of the Study:

  • To provide a detailed protocol for fabricating and conditioning tetrodes.
  • To describe the installation of tetrodes into a micro-drive array for chronic in-vivo recording.
  • To outline methods for ground wire connection and protective cone attachment.

Main Methods:

  • Fabrication and conditioning of tetrodes using basic laboratory tools.

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Micro-drive Array for Chronic in vivo Recording: Drive Fabrication
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Micro-drive Array for Chronic in vivo Recording: Drive Fabrication

Published on: April 20, 2009

Construction of Microdrive Arrays for Chronic Neural Recordings in Awake Behaving Mice
10:44

Construction of Microdrive Arrays for Chronic Neural Recordings in Awake Behaving Mice

Published on: July 5, 2013

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Last Updated: Jun 23, 2026

Micro-drive Array for Chronic in vivo Recording: Tetrode Assembly
14:19

Micro-drive Array for Chronic in vivo Recording: Tetrode Assembly

Published on: April 22, 2009

Micro-drive Array for Chronic in vivo Recording: Drive Fabrication
14:03

Micro-drive Array for Chronic in vivo Recording: Drive Fabrication

Published on: April 20, 2009

Construction of Microdrive Arrays for Chronic Neural Recordings in Awake Behaving Mice
10:44

Construction of Microdrive Arrays for Chronic Neural Recordings in Awake Behaving Mice

Published on: July 5, 2013

  • Assembly of tetrodes into a multi-drive array for chronic implantation in rats.
  • Stereotaxic surgery for precise tetrode placement and micro-drive array integration.
  • Attachment of a protective cone to shield tetrodes during recording.

    • Main Results:

    • Successful fabrication and conditioning of tetrodes suitable for in-vivo recording.
    • Demonstration of precise tetrode placement and stable control using a micro-drive array.
    • Establishment of reliable ground wire connections for signal integrity.
    • Implementation of a protective cone to enhance tetrode durability.

    Conclusions:

    • This protocol offers a reproducible method for preparing and implanting tetrodes for chronic neural recordings.
    • The described techniques facilitate long-term, stable recording of neural activity in freely moving rats.
    • This approach supports investigations into the neural basis of behavior and brain function over extended periods.