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Updated: Jun 19, 2026

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
Published on: March 23, 2015
Functions of mitochondria: from intracellular power stations to mediators of a senescence program
1Belozersky Institute of Physico-Chemical Biology, Moscow State University, Vorobyevy Gory, Bldg. 40, Moscow, 119991, Russia. skulach@belozersky.msu.ru
Abstract:
In 1950 s I started in science by showing that non-phosphorylating respiration is critical for survival of an animal at low temperature. Later, in the 1960 s and 1970 s, I took part in verification of Mitchell's chemiosmotic hypothesis postulating that (i)mitochondria transform energy of respiration to electricity and (ii) uncoupling of respiration represents discharge of this electricity by H(+) cycling. Fifteen years ago I turned to a specific kind of mitochondrial respiration which produces O(2)(-.), and I came to the conclusion that it plays an ominous role, killing mitochondria, cells, or even organisms. My present task is a "megaproject" with an ambitious goal of minimizing the damaging effect of O(2)(-.) and stopping senescence.
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