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Updated: Jun 23, 2026

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
Published on: March 14, 2021
Characterisation of RC-proteoliposomes at different RC/lipid ratios
Francesco Milano1, Francesca Italiano, Angela Agostiano
1CNR, Istituto per i Processi Chimico-Fisici, Sezione di Bari, c/o Dipartimento di Chimica, Via Orabona, 4 I-70124, Bari, Italy.
Abstract:
Reconstitution of membrane proteins in phospholipid vesicles allows the investigation of such macromolecules in a biomimetic simplified environment. The often employed micelle-to-vesicle-transition method for proteoliposome preparation is a fast and reproducible technique. In this, communication is shown that the lipid/protein ratio influences the size of the proteoliposomes and the actual protein reconstitution. The results indicate that for photosynthetic reaction centres, the best conditions for ligand-interaction experiments are achieved with a lipid/protein value of 1000:1, while for complete protein incorporation, the 2000:1 ratio should be chosen.
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