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Synergistic inhibition effect of tumor growth by using celecoxib in combination with oxaliplatin
Shipeng Zhao1, Jianhui Cai, Honglei Bian
1Department of Coloproctology, Third Hospital of Hebei Medical University, Shijiazhuang HeBei, China. shipengzcn@yahoo.com.cn
Background:
To evaluate the effects of celecoxib, in combination with oxaliplatin, on tumour growth, cell apoptosis and angiogenesis in nude mice models.
Materials And Methods:
Xenograft mice model of colon cancer was established using the BALB/c nude mice. Medicine was administered respectively in different groups. Tumour volumes, the expression level of survivin, beta-catenin, VEGF mRNA level, the microvessel density and cell apoptosis were measured.
Results:
Celecoxib, oxaliplatin and combination use caused a tumour inhibition of 35%, 31% and 63% respectively. Compared with control group, a significant redation of angiogenesis and the levels of COX-2, survivin and beta-cantenin protein, and increase of cell apoptosis were detected in tumors in celecoxib group. Celecoxib in combination with oxaliplatin could further promote cell apoptosis and reduce beta-catenin protein expression.
Conclusion:
Celecoxib could inhibit tumor growth and enhance the antitumor effects of oxaliplatin through their synergistic role in inhibiting different targets.
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