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Preparation Of Neovascular Tissues from Human Glioma Tissues for Quantitative Proteomics Analysis of Tumor Angiogenesis
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Thrombin enhances glioma growth
Ya Hua1, Lingling Tang, Richard F Keep
1Department of Neurosurgery, University of Michigan, Room 5018, BSRB, Ann Arbor, MI 48109-2200, USA. yahua@umich.edu
Acta Neurochirurgica. Supplement
|April 25, 2009
Summary
Thrombin significantly enhances glioma growth in rats and increases glioma cell proliferation in vitro. This suggests thrombin may be a potential therapeutic target for glioma treatment.
Area of Science:
- Neuro-oncology
- Molecular Biology
- Pharmacology
Background:
- Previous studies show argatroban reduces brain edema in rat glioma models.
- This study investigates thrombin's role in glioma growth.
Purpose of the Study:
- To determine if thrombin enhances glioma growth in vivo.
- To assess thrombin's effect on glioma cell proliferation in vitro.
Main Methods:
- Rats received intracerebral C6 glioma cells treated with or without thrombin.
- In vitro, C6 cells were treated with thrombin and/or argatroban.
- DNA synthesis (BrdU ELISA) and cell proliferation (MTT assay) were measured.
Main Results:
- Thrombin-treated C6 cells led to larger tumors and worsened neurological deficits in rats.
- In vitro, thrombin increased glioma cell DNA synthesis and proliferation.
- Argatroban, a thrombin inhibitor, blocked thrombin's effect on DNA synthesis.
Conclusions:
- Thrombin promotes glioma growth in vivo and proliferation in vitro.
- Thrombin inhibition may represent a novel therapeutic strategy for glioma.
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