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Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation
Published on: May 23, 2025
Membrane array-based differential profiling of platelets during storage for 52 miRNAs associated with apoptosis
Meganathan Kannan1, Ketha V Krishna Mohan, Sandhya Kulkarni
1Section of Cell Biology, Laboratory of Cellular Hematology, Center for Biologics Evaluation and Research, Food and Drug Administration/PHS, Bethesda, Maryland.
Transfusion
|April 25, 2009
Summary
Platelet storage lesions alter microRNA (miRNA) profiles. Specific miRNAs, like Let-7b and miR-16, increase while others decrease, offering insights into platelet storage.
Area of Science:
- Biochemistry
- Molecular Biology
- Hematology
Background:
- Enucleated platelets (PLTs) rely on posttranscriptional gene regulation (PTGR) for function.
- Ex vivo platelet storage leads to storage lesions, altering PLT functions.
- Apoptosis-associated microRNAs (miRNAs) role in PLT storage lesions is not fully understood.
Purpose of the Study:
- To comprehensively analyze apoptosis-associated miRNA profiles in stored platelets.
- To identify specific miRNAs that change during platelet storage.
- To investigate potential miRNA-target mRNA interactions in platelet storage lesions.
Main Methods:
- Differential profiling of 52 apoptosis-associated human miRNAs in room temperature-stored PLTs (Days 0, 2, 9).
- Total RNA extraction followed by membrane array-based miRNA analysis.
- Bioinformatics approaches for target gene prediction.
Main Results:
- Let-7a, -7c, -7e, -7f, -7g, and -7i miRNA levels were significantly decreased during storage.
- miR-150, -151, -152, -184, -188, -196a, -197, and -202 remained at high levels.
- Let-7b and miR-16 showed an increasing trend, while miR-7 and miR-145 decreased during storage. Potential target mRNAs were identified for these four miRNAs.
Conclusions:
- This study confirms the presence of miRNAs in platelets.
- A subset of apoptosis-specific miRNAs are perturbed during platelet storage.
- Further research on miRNA-target mRNA interactions is crucial for understanding PLT storage lesions.
