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Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae
07:37

Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae

Published on: April 30, 2018

Thrombin and interleukin-1beta decrease HOX gene expression in human first trimester decidual cells: implications for

Jennifer Sarno1, Frederick Schatz, S Joseph Huang

  • 1Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, CT 06520, USA.

Molecular Human Reproduction
|April 25, 2009
PubMed
Summary
This summary is machine-generated.

Bleeding and inflammation in early pregnancy reduce crucial HOX gene expression (HOXA10, HOXA11) in the decidua. This may explain how these pregnancy complications lead to pregnancy loss.

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Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae
07:37

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Published on: April 30, 2018

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08:19

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Published on: May 21, 2015

Area of Science:

  • Reproductive biology
  • Developmental biology
  • Genetics

Background:

  • Early pregnancy bleeding or inflammation can cause pregnancy loss or implantation defects.
  • The impact of these conditions on HOX gene expression in the first trimester decidua is not well understood.

Purpose of the Study:

  • To investigate the effect of thrombin and Interleukin-1beta (IL-1beta) on HOX gene expression in human first-trimester decidual cells.
  • To compare HOX gene expression in decidua from spontaneous pregnancy loss versus viable pregnancies.

Main Methods:

  • First-trimester decidual cells were treated with hormones (estradiol, medroxyprogesterone acetate) followed by thrombin or IL-1beta.
  • Gene expression was analyzed using Affymetrix microarray and confirmed with real-time RT-PCR.
  • HOX gene RNA and protein levels were assessed in decidua from pregnancy loss and viable pregnancies.

Main Results:

  • Hormonal treatment increased HOXA10 and HOXA11 expression.
  • Thrombin significantly decreased HOXA10 and HOXA9 expression.
  • IL-1beta significantly decreased HOXA1, HOXA3, HOXA9, HOXA10, and HOXA11 expression.
  • Decidua from spontaneous pregnancy loss showed reduced HOXA10 and HOXA11 RNA and protein compared to viable pregnancies.

Conclusions:

  • Decidual cells express multiple HOX genes, which are inhibited by thrombin and IL-1beta.
  • Reduced expression of HOXA10 and HOXA11, essential for successful pregnancy, may be a mechanism linking bleeding/inflammation to adverse pregnancy outcomes.