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Related Experiment Videos

Experimental models of plasma perfusion.

C D Pusey1, M Aslam, J Cohen

  • 1Department of Medicine, Royal Postgraduate Medical School, London, England.

Journal of Clinical Apheresis
|January 1, 1991
PubMed
Summary

A new system enables repeated plasma perfusion in conscious rats for disease treatment research. This method effectively reduced harmful substances in models of autoimmune disease, endotoxemia, and liver failure.

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Area of Science:

  • Nephrology
  • Toxicology
  • Hepatology

Background:

  • Developing effective treatments for complex diseases requires advanced research models.
  • Plasma filtration with sorbent treatment offers a potential therapeutic strategy for various conditions.

Purpose of the Study:

  • To develop and evaluate a system for repeated plasma perfusion in unrestrained conscious rats.
  • To assess the efficacy of on-line sorbent treatment in preclinical disease models.

Main Methods:

  • Developed a chronic plasma perfusion system in conscious rats.
  • Utilized specific sorbent columns targeting disease-related substances (e.g., anti-GBM antibodies, endotoxins).
  • Evaluated outcomes in experimental autoimmune glomerulonephritis, endotoxemia, and acute hepatic failure models.

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Main Results:

  • Perfusion over glomerular basement membrane antigen columns reduced anti-GBM antibodies in glomerulonephritis.
  • Polymyxin B perfusion lowered endotoxin levels and improved survival in endotoxemia.
  • Combined sorbent columns enhanced survival in hepatic coma associated with acute liver failure.

Conclusions:

  • The developed plasma perfusion system is effective for preclinical evaluation of sorbent therapies.
  • On-line sorbent treatment shows promise for managing autoimmune diseases, endotoxemia, and acute liver failure.
  • This system facilitates the study of various sorbents for treating a range of disorders.