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Survival of Prevotella intermedia (Bacteroides intermedius) in transport media
E Deveaux1, P Delplanque, C Neut
1Département d'Odontologie Conservatrice-Endodontie, U.F.R. d'Odontologie, Université de Lille II, France.
Abstract:
Five transport media were selected for testing, in vitro, the survival of a pure strain of Prevotella intermedia (Bacteroides intermedius) for 6, 24, and 72 hours. Two were non-nutrient transport media (RTF and VMG IV). The three others (TG, PY, PYG) were nutrient media. An increase in the transport time, and manipulations in an aerobic atmosphere compromised the survival of the bacteria. PY ensured good survival of the bacteria. RTF seems to be the best medium for the transport of P. intermedia, when quantitative analysis is required.
Insights
Prevotella intermedia survival varied across five transport media. Nutrient media like PY supported bacterial survival, while RTF proved optimal for quantitative Prevotella intermedia transport.
Area of Science:
- Microbiology
- Bacteriology
Background:
- Prevotella intermedia (formerly Bacteroides intermedius) is a significant oral bacterium.
- Effective transport media are crucial for accurate microbiological analysis and maintaining bacterial viability.
Purpose of the Study:
- To evaluate the in vitro survival of Prevotella intermedia in five different transport media.
- To determine the impact of transport time and atmospheric conditions on bacterial survival.
Main Methods:
- Five transport media (RTF, VMG IV, TG, PY, PYG) were tested.
- Survival of Prevotella intermedia was assessed at 6, 24, and 72 hours.
- Experiments involved both nutrient and non-nutrient media under varying atmospheric conditions.
Main Results:
- Bacterial survival decreased with increased transport time and exposure to aerobic conditions.
- Nutrient media, particularly PY, demonstrated good survival rates for Prevotella intermedia.
- RTF medium was identified as the most suitable for transporting Prevotella intermedia when quantitative analysis is intended.
Conclusions:
- Transport medium choice significantly impacts Prevotella intermedia viability.
- RTF medium is recommended for quantitative transport of Prevotella intermedia.
- Aerobic conditions and prolonged transport times negatively affect bacterial survival.