Related Experiment Videos
Isolation and characterization of a Saccharomyces cerevisiae mutant disrupted for the succinate dehydrogenase
K M Robinson1, A von Kieckebusch-Gück, B D Lemire
1Department of Biochemistry, University of Alberta, Edmonton, Canada.
Abstract:
A partial genomic clone of the flavoprotein subunit of the mitochondrial enzyme, succinate dehydrogenase (EC 1.3.99.1) from Saccharomyces cerevisiae has been isolated. The partial clone was used to construct, by targeted gene disruption, a yeast mutant with a defective flavoprotein subunit gene. Submitochondrial membranes from the mutant are defective in activities requiring a functional succinate dehydrogenase but not in other respiratory chain activities. In addition, the mutant contains significantly lower levels of covalently attached flavin adenine dinucleotide cofactor than does the wild type. Disruption of the flavoprotein subunit gene results in the simultaneous loss of both the iron-sulfur and the flavoprotein subunits from mitochondrial membranes.