Phase diagram of single vesicle dynamical states in shear flow

We report the first experimental phase diagram of vesicle dynamical states in a shear flow presented in a space of two dimensionless parameters suggested recently by V. Lebedev et al. To reduce errors in the control parameters, 3D geometrical reconstruction and determination of the viscosity contrast of a vesicle in situ in a plane Couette flow device prior to the experiment are developed. Our results are in accord with the theory predicting three distinctly separating regions of vesicle dynamical states in the plane of just two self-similar parameters.