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Related Experiment Video

Updated: Jun 23, 2026

qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes
07:58

qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes

Published on: March 6, 2019

A novel duplex SSP-PCR typing method for KIR gene profiling.

E Ashouri1, A Ghaderi, E F Reed

  • 1UCLA Immunogenetics Center, Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, University of California at Los Angeles, Los Angeles, CA 90095-1652, USA.

Tissue Antigens
|April 28, 2009
PubMed
Summary

A new duplex SSP-PCR assay accurately genotypes Killer-cell immunoglobulin-like receptors (KIR) genes. This method simplifies KIR gene content analysis, offering a cost-effective solution for high-volume testing.

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Area of Science:

  • Immunogenetics
  • Molecular Biology

Background:

  • Killer-cell immunoglobulin-like receptors (KIR) are crucial for natural killer cell function.
  • KIR gene content exhibits significant inter-individual variability.
  • Current sequence-specific primer-directed polymerase chain reaction (SSP-PCR) genotyping is laborious and prone to errors.

Purpose of the Study:

  • To develop a simplified and efficient duplex SSP-PCR assay for KIR genotyping.
  • To accurately identify the presence/absence of 16 KIR genes.
  • To distinguish specific KIR2DS4 and KIR3DP1 alleles.

Main Methods:

  • Development of a duplex SSP-PCR assay.
  • Validation using a blind panel of 78 reference DNA standards from the UCLA KIR Exchange Program.
  • Comparison with conventional SSP typing methods.

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Last Updated: Jun 23, 2026

qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes
07:58

qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes

Published on: March 6, 2019

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping
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qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

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Main Results:

  • The duplex SSP-PCR assay demonstrated 100% specificity and accuracy.
  • The assay successfully identified the presence and absence of 16 KIR genes.
  • Subsets of KIR2DS4 and KIR3DP1 alleles were distinguished.

Conclusions:

  • The developed duplex SSP-PCR assay is an accurate, simple, and cost-effective method for KIR genotyping.
  • This assay is suitable for high-volume testing, saving labor and resources.
  • It offers an improvement over conventional SSP typing methods for KIR gene content analysis.