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Updated: Jun 23, 2026

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Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
Published on: September 3, 2009
DOGSET: pre-designed primer sets for fine-scale mapping and DNA sequence interrogation in the dog
1Veterinary Genetics Laboratory, School of Veterinary Medicine, University of California, Davis, CA 95616, USA. akwwong@ucdavis.edu
Animal Genetics
|April 28, 2009
Summary
DOGSET offers a comprehensive online database of computationally mined primer sequences for genetic mapping and gene discovery. This resource aids researchers in targeted re-sequencing and mutation analysis using custom primer designs.
Area of Science:
- Genomics
- Bioinformatics
Background:
- Primer design is crucial for genetic analysis.
- Computational methods can accelerate primer discovery.
Purpose of the Study:
- To introduce DOGSET, an online resource for primer sequences.
- To provide primers for genetic mapping and gene element analysis.
Main Methods:
- Computationally mining primer sequences from a reference genome.
- Utilizing heuristic algorithms for sequence identification.
- Developing a graphical user interface for region selection.
Main Results:
- Access to 32,135 PCR primers for genetic mapping.
- Inclusion of 334,657 sequence-tagged gene elements for re-sequencing.
- Capability to export customized primer designs tailored to wet bench protocols.
Conclusions:
- DOGSET facilitates efficient primer discovery for genetic research.
- The resource supports targeted re-sequencing and mutation discovery.
- User-friendly interface enables customized primer design for experimental needs.
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