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Updated: Jun 23, 2026

Tuning Degradation to Achieve Specific and Efficient Protein Depletion
Published on: July 20, 2019
Rapid proteasomal degradation of transcription factor IIB in accordance with F9 cell differentiation
Seiji Shiraishi1, Naomi Tamamura, Misako Jogo
1Department of Biology, Graduate School of Science, Chiba University, Chiba, Japan.
Abstract:
We found that the levels of all general transcription factors (GTFs) for RNA polymerase II decreased in F9 cells when the cells were subjected to a differentiation procedure. Different from other GTFs, decrease of TFIIB during the differentiation was suppressed by addition of a proteasome inhibitor, MG132. The half-life of TFIIB in the differentiated cells was remarkably reduced compared with that in the undifferentiated cells. Moreover, it was demonstrated that TFIIB is a poly-ubiquitinated protein. Results of this study suggest that components of the transcription machinery decreased in accordance with cell differentiation and that TFIIB is specifically and rapidly degraded by the ubiquitin-proteasome pathway.
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