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Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Monitoring Protein Adsorption with Solid-state Nanopores
08:51

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Published on: December 2, 2011

Interrogating single proteins through nanopores: challenges and opportunities.

Liviu Movileanu1

  • 1Department of Physics, Syracuse University, 201 Physics Building, Syracuse, NY 13244-1130, USA. lmovilea@physics.syr.edu

Trends in Biotechnology
|April 28, 2009
PubMed
Summary

Single nanopore probes offer versatile single-molecule analysis for polypeptides, revealing folding, flexibility, and binding. This technology advances nanomedicine for molecular diagnosis and designing new nanostructures.

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Last Updated: Jun 23, 2026

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Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

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Area of Science:

  • Biophysics
  • Nanomedicine
  • Molecular Biology

Background:

  • Single nanopores serve as versatile probes for analyzing polypeptide features.
  • Engineered protein nanopores offer insights into protein transport through transmembrane pores.

Purpose of the Study:

  • To review current knowledge on nanopore probe techniques.
  • To discuss the potential of nanopore technology in nanomedicine and molecular diagnostics.

Main Methods:

  • Utilizing single nanopore probes for single-molecule analysis.
  • Employing engineered protein nanopores to study protein transport.

Main Results:

  • Nanopore probes can reveal polypeptide folding state, flexibility, mechanical stability, binding affinity, and enzymatic activity.
  • Research in this area uncovers biophysical rules governing protein transport through pores.

Conclusions:

  • Nanopore probe techniques are emerging as powerful tools in nanomedicine.
  • This technology facilitates quantitative examination of complex molecular recognition and aids in designing pore-based nanostructures and diagnostic devices.