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Purification of Phleum pratense pollen extract by immunoaffinity chromatography and high-performance ion-exchange
E Bolzacchini1, P Di Gennaro, G Di Gregorio
1Dipartimento di Chimica Organica e Industriale, Università di Milano, Italy.
Journal of Chromatography
|July 12, 1991
Abstract:
Basic allergens of Phleum pratense pollen extract have been purified by either sequence gel filtration-ion-exchange high-performance liquid chromatography (HPIEX) and size-exclusion HPLC or sequence gel filtration-immunoaffinity chromatography and HPIEX. The second procedure seems to be suitable for preparative purposes.