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Updated: Jun 23, 2026
Particle Agglutination Method for Poliovirus Identification
Published on: April 20, 2011
Direct identification of non-polio enteroviruses in residual paralysis cases by analysis of VP1 sequences
Pooneh Rahimi1, H Tabatabaie, Mohammad M Gouya
1Department of Virology, Pasteur Institute of Iran, Tehran, Iran. prahimi@pasteur.ac.ir
Background:
The 66 serotypes of human enteroviruses (EVs) are classified into four species A-D, based on phylogenetic relationships in multiple genome regions. Partial VP(1) amplification and sequence analysis are reliable methods for identifying non-polio enterovirus serotypes, especially in negative cell culture specimens from patients with residual paralysis.
Objectives:
In Iran during the years 2000-2002, there were 29 residual paralysis cases with negative cell (RD, HEp(2) and L(20)B) culture results.
Study Design:
The genomic RNA was extracted from stool specimens from cases of residual paralysis and detected by amplification of the 5'-nontranslated region using RT-PCR with Pan-EV primers. Partial VP(1) amplification by semi-nested RT-PCR (snRT-PCR) and sequence analysis were done.
Results:
Specimens from the 29 culture-negative cases contained echoviruses of six different serotypes.
Conclusions:
The global eradication of wild polioviruses is near and study of non-polio enteroviruses, which can cause poliomyelitis, is increasingly important to understand their pathogenesis. The VP(1) sequences, derived from the snRT-PCR products, allowed rapid molecular analysis of these non-polio strains.
