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Updated: Jun 23, 2026

Clean Sampling and Analysis of River and Estuarine Waters for Trace Metal Studies
Published on: July 1, 2016
Mercury(II) and methyl mercury determinations in water and fish samples by using solid phase extraction and cold
Mustafa Tuzen1, Isa Karaman, Demirhan Citak
1Chemistry Department, Faculty of Science and Arts, Gaziosmanpaşa University, 60250 Tokat, Turkey.
Abstract:
A method has been developed for mercury(II) and methyl mercury speciation on Staphylococcus aureus loaded Dowex Optipore V-493 micro-column in the presented work, by using cold vapour atomic absorption spectrometry. Selective and sequential elution with 0.1 molL(-1) HCl for methyl mercury and 2 molL(-1) HCl for mercury(II) were performed at the pH range of 2-6. Optimal analytical conditions including pH, amounts of biosorbent, sample volumes were investigated. The detection limits of the analytes were 2.5 ngL(-1) for Hg(II) and 1.7 ngL(-1) for methyl mercury. The capacity of biosorbent for mercury(II) and methyl mercury was 6.5 and 5.4 mgg(-1), respectively. The validation of the presented procedure is performed by the analysis of standard reference material. The speciation procedure established was successfully applied to the speciation of mercury(II) and methyl mercury in natural water and microwave digested fish samples.
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