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Purification of carbonic anhydrase isoenzymes by high-performance affinity chromatography and hydrophobic interaction
H Abou-Rebyeh1, D Josić, K Gottschall
1Institut für Molekularbiologie und Biochemie der Freien Universität Berlin, Germany.
Abstract:
Isoenzymes of carbonic anhydrase were purified by a combination of affinity chromatography and hydrophobic interaction chromatography. Immobilization of sulfonamides on an epoxy-activated support provided a stationary phase for affinity chromatography which was stable to hydrolysis by carbonic anhydrase. A first purification step allowed the isolation of enzymes directly from homogenates of human erythrocytes and rat stomach. Without any further preparation, except the addition of ammonium sulfate to the eluate from affinity chromatography, the isoenzymes could be separated by hydrophobic interaction chromatography with very high recovery of protein and retention of enzymatic activity.