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Updated: Jun 23, 2026

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
Published on: October 15, 2018
2'-deoxy-1-methylpseudocytidine, a stable analog of 2'-deoxy-5-methylisocytidine
Hyo-Joong Kim1, Nicole A Leal, Steven A Benner
1Westheimer Institute for Science and Technology, Foundation for Applied Molecular Evolution, PO Box 13174, Gainesville, FL 32604, USA.
Abstract:
2'-deoxy-5-methylisocytidine is widely used in assays to personalize the care of patients infected with HIV, hepatitis C, and other infectious agents. However, oligonucleotides that incorporate 2'-deoxy-5-methylisocytidine are expensive, because of its intrinsic chemical instability. We report here a C-glycoside analog that is more stable and, in oligonucleotides, pairs with 2'-deoxyisoguanosine, contributing to duplex stability about as much as a standard 2'-deoxycytidine and 2'-deoxyguanosine pair.
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