Capillary zone electrophoresis: its applicability and potential in biochemical analysis

Area of Science:

• Analytical Chemistry
• Biochemistry
• Separation Science

Background:

• Capillary zone electrophoresis (CZE) is a powerful separation technique.
• Advances in instrumentation and detection have expanded its capabilities.
• Hyphenation with other techniques enhances analytical power.

Purpose of the Study:

• To review recent developments in capillary zone electrophoresis (CZE).
• To discuss various operational modes and detection systems.
• To highlight applications in biomolecule and chiral separations.

Main Methods:

• Review of current CZE instrumentation and operational modes.
• Description of common detection systems used in CZE.
• Discussion of CZE coupled with mass spectrometry (CZE-MS) and chromatography.

Main Results:

• Overview of advancements in CZE technology and methodologies.
• Exploration of CZE-MS and hyphenated techniques for complex analyses.
• Demonstration of CZE's utility in separating chiral molecules, amino acids, peptides, proteins, and nucleic acids.

Conclusions:

• CZE continues to evolve with improved instrumentation and coupling techniques.
• CZE is a versatile tool for analyzing a wide range of biomolecules and chiral compounds.
• Further integration with other analytical methods promises broader applications.