Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic technologies to enhance crop nutritional value under climate change.

Nature·2026
Same author

Granular datasets will be key to predicting the impact of food system transitions on micronutrient status in China.

Nature food·2026
Same author

Comparative genome analysis provides a foundation for defining salvinorin A biosynthesis in Salvia divinorum.

Nature communications·2026
Same author

A dirigent protein redirects extracellular terpenoid metabolism for defense against biotic challenges.

Nature communications·2025
Same author

Effect of Vitamin D2 Supplementation on 25-Hydroxyvitamin D3 Status: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.

Nutrition reviews·2025
Same author

Two Stabiliser loci suppress Tam3 transposition without compromising transposase production in Antirrhinum.

Plant physiology·2025
Same journal

The gatekeeper: How PSY1 controls root growth.

The Plant cell·2026
Same journal

OsOSCA2.4 regulates post-Golgi trafficking of storage proteins by modulating Ca2+ homeostasis in rice endosperm.

The Plant cell·2026
Same journal

Age-dependent epigenetic control of flavonoid metabolism underlies chemical defenses in ancient Ginkgo biloba.

The Plant cell·2026
Same journal

The CDC48A-PUX10 Complex Regulates Peroxisomal Protein Homeostasis via Ubiquitin-Dependent Degradation in Arabidopsis.

The Plant cell·2026
Same journal

VvNUP96-VvTCP15 coupling regulates growth and salt tolerance via a VvmiR319c-VvTCP4 module in grapevine.

The Plant cell·2026
Same journal

CitNAC71-CitNAC76 coordinate cellulose and hemicellulose biosynthesis to regulate high-temperature-mediated granulation in citrus.

The Plant cell·2026
See all related articles

Related Experiment Video

Updated: Jun 23, 2026

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR
07:06

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR

Published on: February 6, 2026

Guidelines for quantitative rt-PCR

Cathie Martin1

  • 1Editor in Chief.

The Plant Cell
|April 28, 2009
PubMed
Summary

No abstract available in PubMed .

More Related Videos

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

Sublingual Immunotherapy as an Alternative to Induce Protection Against Acute Respiratory Infections
16:56

Sublingual Immunotherapy as an Alternative to Induce Protection Against Acute Respiratory Infections

Published on: August 30, 2014

Related Experiment Videos

Last Updated: Jun 23, 2026

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR
07:06

Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR

Published on: February 6, 2026

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
10:28

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

Sublingual Immunotherapy as an Alternative to Induce Protection Against Acute Respiratory Infections
16:56

Sublingual Immunotherapy as an Alternative to Induce Protection Against Acute Respiratory Infections

Published on: August 30, 2014