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Related Experiment Video

Updated: Jun 23, 2026

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

Moonlighting proteins--an update.

Constance J Jeffery¹

¹Laboratory for Molecular Biology, Department of Biological Sciences, MC567, University of Illinois, 900 S. Ashland Ave., Chicago, IL 60607, USA. cjeffery@uic.edu

Molecular Biosystems

|April 28, 2009

Summary

This summary is machine-generated.

Discover moonlighting proteins, single proteins with multiple functions, excluding gene fusions or splice variants. This review highlights new discoveries and structural insights into these fascinating multifunctional proteins.

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Area of Science:

Biochemistry
Molecular Biology
Proteomics

Background:

Moonlighting proteins are single polypeptide chains performing distinct functions.
They are distinct from multifunctional proteins arising from gene fusions, homologous families, splice variants, or promiscuous activities.
Recent research expands the known repertoire of these unique proteins.

Purpose of the Study:

To review recent discoveries of moonlighting proteins.
To summarize new insights into their molecular mechanisms and functional switching.
To discuss the potential for intrinsically unstructured proteins to moonlight.

Main Methods:

Literature review of recent findings on moonlighting proteins.
Analysis of proteomics microarray data identifying novel functions.
Examination of X-ray crystal structures for mechanistic understanding.

Main Results:

Several new moonlighting proteins with diverse functions have been identified.
A biosynthetic enzyme was found to possess a novel DNA binding function.
X-ray crystallography provides structural basis for dual functions and inter-functional switching.

Conclusions:

The diversity of moonlighting proteins continues to grow.
Structural studies are crucial for elucidating their complex mechanisms.
Intrinsically unstructured regions may contribute to moonlighting capabilities.