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Updated: Jun 23, 2026

Functional Assessment of Intestinal Tight Junction Barrier and Ion Permeability in Native Tissue by Ussing Chamber Technique
Published on: May 26, 2021
Molecular mechanism of intestinal permeability: interaction at tight junctions
Zakir Hossain1, Takashi Hirata
1Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
Abstract:
The intestinal barrier plays a critical role in humans in the transport of nutrients and macromolecules. At the same time, it has to provide an effective barrier to harmful macromolecules and microorganisms. The tight junction (TJ) is an essential component of this barrier. The junctional complexes of the plasma membrane are not simply epithelial barriers in paracellular transport or barriers preventing diffusion in the plasma membrane, but also contain proteins involved in signal transduction and the maintenance of the physiological epithelial cell state. Occludin, claudin, junctional adhesion molecules, and the coxsackie virus and adenovirus receptor are the major components of TJs. This article highlights the structure and function of TJs as well as the molecular interactions occurring during permeation through TJs.
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