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[Expression of matrix metalloproteinase-3 after brain contusion in rats]
Xiao-chong Guo1, Ru-bo Li, Hong-xia Liang
1Department of Forensic Pathology, School of Forensic Medicine, China Medical University, Shenyang 110001, China.
Objective:
To investigate the expression of matrix metalloproteinase-3 after brain contusion and its applicability for estimating the age of brain contusion.
Methods:
Rats had been divided into three groups: control group, sham operation group and brain contusion group. The expression of matrix metalloproteinase-3 at different time was detected by immunohistochemistry and Western blot.
Results:
By the immunohistochemistry, no staining was observed in control and sham operation groups. The positive staining of MMP-3 appeared 6 hours after contusion, increased gradually in 24 hours and peaked 5 days after contusion, then started to decrease, 14 days after contusion still could be observed. By the Western blot analysis, no expression of MMP-3 was detected in control and sham groups. The positive staining of MMP-3 appeared 6 hours after contusion, increased gradually and maximized 5 days after contusion, then started to decrease, 14 days after contusion still could be found.
Conclusion:
Time-order expression of MMP-3 could be used for estimating the age of brain contusion in forensic pathology.
