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Published on: July 17, 2021
[The application of three methods combined for extracting DNA from human epithelium]
Yan-xia Guo1, Song Chen, Kai-hui Liu
1Forensic Science Institute of Public Security Ministry, Beijing 100038, China.
Objective:
To improve DNA extraction from human epithelium.
Methods:
Chelex-100, Chelex-100 organic combined and Chelex-100 magnetism pearl combined methods were adopted according to the situation of each case.
Results:
With the stepwise extracting protocol, the trace amount of DNA was analyzed most efficiently and the success rate of DNA extracting was better improved.
Conclusion:
The efficiency of DNA extraction from human epithelium could be greatly improved by modifying the extracting protocol such as purification and concentration based on previous results.
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