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Core-cross-linked alginate microcapsules for cell encapsulation.

M A Jafar Mazumder1, Nicholas A D Burke, Feng Shen

  • 1Department of Chemistry, McMaster University, Hamilton, Ontario, Canada.

Biomacromolecules
|April 29, 2009
PubMed
Summary

New self-cross-linkable polyelectrolytes create robust, cyto-compatible CaAlg beads for cell encapsulation. This method offers potential for cell immuno-isolation in therapeutic transplants.

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Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Cell Encapsulation Technology

Background:

  • Alginate (Alg) beads are widely used for cell encapsulation but often lack robust mechanical and chemical stability.
  • Developing stable, biocompatible encapsulation systems is crucial for therapeutic cell transplantation.

Purpose of the Study:

  • To develop novel self-cross-linkable polyelectrolyte systems for enhanced calcium alginate (CaAlg) bead stability.
  • To create cyto-compatible CaAlg beads with tunable cross-linking for cell immuno-isolation.

Main Methods:

  • Incorporation of self-cross-linkable polyelectrolyte pairs, poly(methacrylic acid, sodium salt-co-2-[methacryloyloxy]ethyl acetoacetate) (A70) and poly-L-lysine, into CaAlg beads.
  • Formation of either a covalently cross-linked shell or a core-cross-linked bead by controlling poly-L-lysine molecular weight.

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  • Utilizing a calcium chloride gelling bath for bead formation and subsequent cross-linking steps.

    • Main Results:

    • Achieved covalently cross-linked CaAlg beads with either a shell or core structure.
    • Demonstrated resistance to chemical and mechanical stress while maintaining cyto-compatibility.
    • Tunable cross-linking achieved using different molecular weights of poly-L-lysine (15-30 kDa for shell, 4-15 kDa for core).

    Conclusions:

    • The novel polyelectrolyte system provides a robust and cyto-compatible method for CaAlg bead formation.
    • This approach enables versatile cell encapsulation with potential applications in therapeutic cell transplants for immuno-isolation.