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Updated: Jun 23, 2026
07:43
Assaying Proteasomal Degradation in a Cell-free System in Plants
Published on: March 26, 2014
Targeting the proteasome pathway.
Sachiko Tsukamoto1, Hideyoshi Yokosawa
1Chiba University, Graduate School of Science, 1-33 Yayoi-cho, Inage-ku, Chiba 263 8522, Japan.
Expert Opinion on Therapeutic Targets
|April 29, 2009
Summary
The ubiquitin-proteasome pathway is crucial for protein degradation. Inhibitors targeting this pathway, like proteasome inhibitors, show promise as cancer therapies.
Area of Science:
- Biochemistry
- Molecular Biology
- Pharmacology
Background:
- The ubiquitin-proteasome pathway is essential for intracellular protein degradation, impacting numerous cellular processes.
- Inhibitors of this pathway are being developed, with bortezomib (Velcade) approved for multiple myeloma treatment.
- This highlights the therapeutic potential of targeting the ubiquitin-proteasome system.
Purpose of the Study:
- To review the current understanding of the ubiquitin-proteasome pathway.
- To discuss inhibitors targeting this pathway, including proteasome inhibitors.
- To evaluate their potential as candidate drugs for chemical therapy.
Main Methods:
- Review of preclinical data for ubiquitin-proteasome pathway inhibitors.
- Review of clinical data for ubiquitin-proteasome pathway inhibitors.
- Discussion of proteasome inhibitors and their therapeutic applications.
Main Results:
- The ubiquitin-proteasome pathway plays a critical role in cellular functions.
- Various inhibitors targeting this pathway have been investigated.
- Proteasome inhibitors have demonstrated therapeutic efficacy.
Conclusions:
- The proteasome is a validated therapeutic target.
- Other components of the ubiquitin-proteasome pathway are emerging as novel targets.
- Targeting the ubiquitin-proteasome pathway offers promising avenues for cancer therapy.
Related Concept Videos
02:18The Proteasome
Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
01:13The Proteasome
Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3 (ubiquitin...
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3 (ubiquitin...
02:18The Proteasome
Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
01:17The Proteasome Structure
The ubiquitin-proteasome pathway is a well-known mechanism utilized by eukaryotic cells to remove cytoplasmic proteins that are misfolded, damaged, or no longer needed. In this pathway, the protein that needs to be eliminated undergoes a process called ubiquitination, where a chain of ubiquitin molecules is attached to the 48th lysine residue of the target protein. This ubiquitin modification helps the proteasome distinguish between a target protein and a healthy protein.
The proteasome is an...
The proteasome is an...
02:58Regulated Protein Degradation
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
02:58Regulated Protein Degradation
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
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