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High-Performance Liquid Chromatography: Introduction

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
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Related Experiment Video

Updated: Jun 23, 2026

Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults

Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults

Published on: April 22, 2022

Comprehensive two-dimensional liquid chromatography to quantify polyphenols in red wines.

Paola Dugo¹, Francesco Cacciola, Paola Donato

¹Department of Food and Environmental Science, University of Messina, Salita Sperone 31, 98166 Messina, Italy. pdugo@pharma.unime.it

Journal of Chromatography. A

|April 29, 2009

Summary

Comprehensive two-dimensional liquid chromatography (2D-LC) offers superior polyphenol quantification in red wine compared to conventional methods. This advanced technique enhances compound detection and reduces matrix interferences for more accurate wine analysis.

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Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults

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Published on: June 10, 2022

Area of Science:

Analytical Chemistry
Food Science
Chromatography

Background:

Quantification of polyphenols in complex matrices like red wine is challenging with conventional methods.
Comprehensive two-dimensional liquid chromatography (2D-LC) offers enhanced separation capabilities but is rarely used for quantification.
Polyphenols are key compounds in red wine, influencing its health benefits and sensory properties.

Purpose of the Study:

To apply and validate a comprehensive 2D-LC method for quantifying polyphenols in red wines.
To compare the performance of comprehensive 2D-LC with conventional one-dimensional LC for polyphenol analysis.
To assess the suitability of comprehensive 2D-LC for analyzing complex natural samples with minimal pre-treatment.

Main Methods:

Utilized a comprehensive 2D-LC system with a microbore conventional HPLC column (1D) and a partially porous column (2D).
Employed dedicated software for peak detection and quantification from the 2D chromatogram.
Validated the method for linearity, sensitivity, and limits of detection/quantification.

Main Results:

Comprehensive 2D-LC enabled the quantification of a higher number of polyphenols compared to 1D-HPLC-DAD.
Improved resolving power of 2D-LC led to relative standard deviations (RSD) below 10% for quantified compounds.
The method allowed analysis of red wine samples with minimal pre-treatment, reducing matrix interferences.

Conclusions:

Comprehensive 2D-LC is a powerful tool for accurate and sensitive polyphenol quantification in red wine.
This method offers significant advantages over conventional LC in terms of separation efficiency and the number of quantifiable compounds.
Comprehensive 2D-LC facilitates the analysis of complex natural products without extensive sample preparation.