Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prostate radiotherapy may cause fertility issues: a retrospective analysis of testicular dose following modern radiotherapy techniques.

Radiation oncology (London, England)·2024
Same author

Spontaneous resolution of intracranial hypertension following radiotherapy for posterior parasagittal meningioma: About two cases and review of the literature.

Cancer radiotherapie : journal de la Societe francaise de radiotherapie oncologique·2023
Same author

Immune checkpoint inhibitors-induced systemic capillary leak syndrome: A report of two cases.

La Revue de medecine interne·2022
Same author

Absolute Metabolite Quantification Using <i>Pure Shift</i> NMR: Toward Quantitative Metabolic Profiling of Aqueous Biological Samples.

Analytical chemistry·2022
Same author

[Stereotactic radiotherapy of non-tumoral brain pathologies: Arteriovenous malformations and trigeminal neuralgias].

Cancer radiotherapie : journal de la Societe francaise de radiotherapie oncologique·2022
Same author

[Organ preservation for rectal cancer: What are the arguments in favor of radiotherapy?]

Cancer radiotherapie : journal de la Societe francaise de radiotherapie oncologique·2022
Same journal

Localization-driven exchange contrast in diffusion exchange spectroscopy.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

4.5 Tesla superconducting miniature magnet in liquid nitrogen.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Folding and unfolding dynamics of a DNA aptamer studied by heteronuclear <sup>1</sup>H-<sup>13</sup>C correlation zz-exchange spectroscopy.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Multi-spin control from one-spin pulses.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Altering MRI rotating frame relaxations by changing the truncation level of Hyperbolic Secant pulse.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
Same journal

Effects of proton exchange on the lifetimes of long-lived states in aliphatic chains.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026
See all related articles

Related Experiment Video

Updated: Jun 23, 2026

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
06:25

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Improvements to selective refocusing phased (SERFph) experiments.

L Beguin1, N Giraud, J M Ouvrard

  • 1Laboratoire de RMN en milieu orienté, Université Paris-Sud 11, ICMMO, UMR CNRS 8182, Bat. 410, 91405 Orsay cedex, France.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|April 29, 2009
PubMed
Summary
This summary is machine-generated.

Selective refocusing experiments improve proton-proton coupling analysis. Adding a refocusing pulse and zero-quantum filter enhances sensitivity and provides pure absorption lineshapes for differentiating enantiomers.

More Related Videos

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

Related Experiment Videos

Last Updated: Jun 23, 2026

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
06:25

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
08:39

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
09:13

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Analytical Chemistry
  • Chiroptical Methods

Background:

  • Selective refocusing experiments are crucial for isolating individual proton-proton couplings in complex molecules.
  • Standard experimental sequences can introduce spectral artifacts, complicating data interpretation and reducing sensitivity.

Purpose of the Study:

  • To address spectral artifacts and enhance sensitivity in selective refocusing NMR experiments.
  • To develop an improved methodology for the differentiation of enantiomers using NMR spectroscopy.

Main Methods:

  • Implementation of a refocusing pi pulse combined with a zero-quantum filter in the selective refocusing sequence.
  • Acquisition and analysis of 2D NMR spectra for chiral compounds dissolved in a liquid crystal medium.

Main Results:

  • The combined pulse and filter significantly reduced spectral artifacts, leading to cleaner spectra.
  • Experimental sensitivity was greatly improved, enabling clearer observation of couplings.
  • Pure absorption lineshapes were obtained in the phased 2D spectra, facilitating accurate analysis.

Conclusions:

  • The enhanced NMR methodology provides superior spectral quality and sensitivity for analyzing proton-proton couplings.
  • This technique offers a powerful tool for the differentiation of enantiomers in chiral environments.