Related Experiment Video
Updated: Jun 23, 2026

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces
Published on: January 7, 2020
Inhibition of LPS-induced proinflammatory responses of J774.2 macrophages by immobilized enzymatically tailored
M Gallet1, M Vayssade, M Morra
1UMR CNRS 6600, Biomécanique et Bioingénierie, Université de Technologie de Compiègne, BP 20529, 60205 Compiègne Cedex, France.
Abstract:
The surface of an implant device can be modified by immobilizing biological molecules on it to improve its integration into the host tissue. We have previously demonstrated that enzymatically tailored plant pectins are promising nanocoatings for biomaterials. This study investigates whether a coating of modified hairy region (rhamnogalacturonan-I) from apple pectin (MHR-alpha) which has anti-adhesive properties can inhibit the generation of inflammatory mediators by lipopolysaccharide (LPS)-activated macrophages. For that purpose, J774.2 murine macrophages were cultured for 24h on MHR-alpha-coated Petri dishes and tissue culture polystyrene controls, with and without LPS. Cell morphology, cell growth, nitrite and TNF-alpha secretion were studied. The results indicate that MHR-alpha coating inhibits the LPS-induced activation of macrophages.
Related Concept Videos
Inhibitors of Gram-positive Cell Wall Synthesis
Formation of Lipopolysaccharides
Cellulose and Pectic Polysaccharides
As a cell matures, its cell wall specializes according to its type. For example, the parenchyma cells of...

