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Related Experiment Video

Updated: Jun 23, 2026

Stereological and Flow Cytometry Characterization of Leukocyte Subpopulations in Models of Transient or Permanent Cerebral Ischemia

Stereological and Flow Cytometry Characterization of Leukocyte Subpopulations in Models of Transient or Permanent Cerebral Ischemia

Published on: December 28, 2014

Platelet function following acute cerebral ischemia.

Jonathan Smout¹, Alexander Dyker, Marcus Cleanthis

¹Northern Vascular Centre Freeman Hospital, Northern Vascular Centre, Newcastle Upon Tyne, United Kingdom.

|April 29, 2009

Summary

This summary is machine-generated.

Monocyte platelet aggregates (MPAs) are elevated after acute ischemic stroke, indicating increased platelet activation. This study suggests MPAs are more sensitive markers than P-selectin or aggregation.

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Stereological and Flow Cytometry Characterization of Leukocyte Subpopulations in Models of Transient or Permanent Cerebral Ischemia

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A Thrombotic Stroke Model Based On Transient Cerebral Hypoxia-ischemia

A Thrombotic Stroke Model Based On Transient Cerebral Hypoxia-ischemia

Published on: August 18, 2015

Area of Science:

Cardiovascular Medicine
Hematology
Neurology

Background:

Platelet activation is increased following acute ischemic stroke.
Accurate measures of platelet activation are needed to evaluate antiplatelet agents.

Purpose of the Study:

To assess platelet activation markers in acute ischemic stroke patients.
To compare monocyte platelet aggregates (MPAs), P-selectin, and platelet aggregation as markers of platelet activation.

Main Methods:

Blood samples were collected from 17 acute ischemic stroke patients and 18 stable arteriopaths.
Platelet aggregation, P-selectin, and MPAs were measured using flow cytometry.
Soluble P-selectin and D-dimers were measured by ELISA.

Main Results:

Initial MPA levels were significantly higher in stroke patients compared to stable patients (p=0.04).
No significant changes were observed in P-selectin or platelet aggregation.
D-dimer and fibrinogen levels showed transient elevations post-stroke.

Conclusions:

Monocyte platelet aggregates (MPAs) are elevated following acute ischemic stroke.
MPAs appear to be a more sensitive marker of platelet activation than P-selectin or aggregation.