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Related Experiment Video

Updated: Jun 23, 2026

High Resolution Quantification of Crystalline Cellulose Accumulation in Arabidopsis Roots to Monitor Tissue-specific Cell Wall Modifications
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Published on: May 10, 2016

KORRIGAN1 and its aspen homolog PttCel9A1 decrease cellulose crystallinity in Arabidopsis stems.

Junko Takahashi1, Ulla J Rudsander, Mattias Hedenström

  • 1Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, SLU, Umeå, Sweden.

Plant & Cell Physiology
|April 29, 2009
PubMed
Summary
This summary is machine-generated.

Hybrid aspen PttCel9A1 complements Arabidopsis KOR1 mutants, functioning in cell walls to facilitate cellulose biosynthesis. This process increases non-crystalline cellulose, impacting plant secondary growth.

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Area of Science:

  • Plant biology
  • Cellulose biosynthesis
  • Biochemistry

Background:

  • KORRIGAN1 (KOR1) is a membrane-bound cellulase essential for cellulose biosynthesis.
  • Understanding KOR1 function is crucial for plant secondary growth and cell wall structure.

Purpose of the Study:

  • To investigate the function of the hybrid aspen PttCel9A1, a putative KOR1 ortholog, in Arabidopsis secondary growth.
  • To elucidate the role of PttCel9A1/KOR1 in cellulose biosynthesis and cell wall composition.

Main Methods:

  • Complementation of Arabidopsis kor1-1 mutants with PttCel9A1.
  • Generation and analysis of transgenic Arabidopsis expressing 35S::PttCel9A1.
  • Analysis of KOR1 mutant line irx2-2.
  • In muro visualization of cellulase activity.
  • (13)C solid-state nuclear magnetic resonance (NMR) spectroscopy to determine cellulose characteristics.

Main Results:

  • PttCel9A1 successfully complemented kor1-1 mutants, confirming it as a KOR1 ortholog.
  • Transgenic lines with elevated PttCel9A1/KOR1 showed increased TFA-hydrolyzable glucan and reduced cellulose crystallinity.
  • irx2-2 mutants exhibited reduced Updegraff cellulose and higher cellulose crystallinity compared to wild type.

Conclusions:

  • PttCel9A1/KOR1 activity is localized in cell walls.
  • PttCel9A1/KOR1 facilitates cellulose biosynthesis, promoting the formation of non-crystalline cellulose.
  • This activity influences plant secondary growth and cell wall properties.