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Published on: April 7, 2017
Preparation and recycling of aqueous two-phase systems with pH-sensitive amphiphilic terpolymer PADB
Ning Biao1, Wan Junfen, Cao Xuejun
1State Key Laboratory of Bioreactor Engineering, Dept. of Biochemical Engineering, East China University of Science and Technology, Shanghai, China 200237.
Abstract:
In this study, a novel pH-sensitive terpolymer PADB was synthesized by random terpolymerization of 2-(dimethylamino) ethyl methacrylate, acrylic acid, and butyl methacrylate. The terpolymer PADB could form aqueous two-phase systems (ATPS) with a light-sensitive terpolymer PNBC, which was synthesized in our laboratory, using n-isopropylacrylamide, n-butyl acrylate, chlorophyllin sodium copper salt as monomers. More than 97% of the PADB terpolymer could be recovered by adjusting the pH to isoelectric point (PI) 4.1. The terpolymer PNBC could be recovered by using light radiation at 488 nm, with recovery ratio of 98%. BSA and lysozyme were partitioned in the PNBC-PADB ATPS to examine this new system. It was found that the partition coefficient of BSA and lysozyme could reach 4.46 and 0.49 in the systems, respectively.
