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Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry
Published on: November 5, 2019
In vitro inhibitory effects of disodium cromoglycate on ionic transports involved in sickle cell dehydration
Léonidas Bizumukama1, Alina Ferster, Béatrice Gulbis
1Laboratory of Medical Biochemistry, Institut de Pharmacie, Université Libre de Bruxelles, Bruxelles, Belgium.
Background:
The antiallergic and antiasthmatic drug disodium cromoglycate (DSCG) has also demonstrated an activity against sickle cell disease, but the mechanism of this action still remains unknown.
Methods:
Na(+) and K(+) fluxes were studied in red cells obtained from 9 patients affected with sickle cell disease in the absence or in the presence of 1 mM of DSCG and deoxygenated under an N(2) flow during up to 24 h.
Results:
A significant inhibiting effect of DSCG on the intracellular K(+) exit and the Na(+) entry was observed.
Conclusion:
These results demonstrate that DSCG partially inhibits the abnormal K(+) loss which is implicated in the dehydration of the sickle cell and the stimulation of sickling.
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