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Updated: Jun 23, 2026

Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract
Published on: November 28, 2012
Characteristics of ethylnitrosourea-induced cataracts
Min-Jung Lee1, Jae-Young Kim, Yeun-Jung Kim
1Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korea 21 Project, Oral Science Research Center, College of Dentistry, Yonsei Center of Biotechnology, Yonsei University, Seoul, Korea.
Purpose:
This study analyzed genes associated with the morphology and regulation of ethylnitrosourea (ENU)-induced cataract mouse.
Materials And Methods:
Immunohistochemistry analysis using anti-crystallins and PCNA antibody revealed that the localization pattern of these specific markers differed between the cataractous and wild-type lens epithelium. Two-dimensional electrophoresis and microarray techniques were used to identify the proteins and genes related to ENU-induced cataract.
Results:
A novel ENU-induced mutation in the mouse led to nuclear and cortical opacity of the eye lens at 5 weeks postnatal. This cataract phenotype was similar to that of the zonular-pulverulent type of human cataract. Crystallin proteins and gap-junction genes have relations to the formation of cataract.
Conclusions:
Together, the results suggest that various proteins affect the formation and specific phenotypes of ENU-induced cataract mouse.
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