Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Restriction Enzymes01:11

Restriction Enzymes

Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Drug-Receptor Bonds01:25

Drug-Receptor Bonds

Drug-receptor bonds are formed through various chemical forces when drugs interact with target cells. Covalent bonds, strong and irreversible, are exemplified by DNA-alkylating anticancer agents that inhibit cell division. However, such irreversible drug binding lacks selectivity and can modify the DNA of the surrounding healthy cells. Covalent binding often contributes to tissue toxicity, as seen with chloroform and paracetamol metabolites binding to the liver, causing hepatotoxicity.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Towards supramolecular regenerative medicine using low-molecular-weight gelator hydrogels for stem cell growth.

Biomaterials science·2026
Same author

Biomechanics of the plesiomorphic forelimb of the basal therizinosaur Falcarius.

Anatomical record (Hoboken, N.J. : 2007)·2026
Same author

When Reality Defies Prediction: Polymorphism, Twinning, and Accordion Crystals.

Journal of the American Chemical Society·2026
Same author

Genomic features associated with sustained mammalian transmission of avian influenza A viruses.

Nature microbiology·2026
Same author

Molecular-Scale Tuning of Low-Molecular-Weight Gelators Controls Supramolecular Assembly and Directs Human Mesenchymal Stem Cell Growth.

Angewandte Chemie (International ed. in English)·2026
Same author

Supramolecular Diversity in Bis(acylhydrazone) Crystals: Linker Effects, Polymorphism, and Gelator Assemblies.

Crystal growth & design·2026

Related Experiment Video

Updated: Jun 23, 2026

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
07:16

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

Published on: February 9, 2024

"On-off" multivalent recognition: degradable dendrons for temporary high-affinity DNA binding.

Daniel J Welsh1, Simon P Jones, David K Smith

  • 1Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK.

Angewandte Chemie (International Ed. in English)
|April 30, 2009
PubMed
Summary
This summary is machine-generated.

Researchers developed a method to switch DNA binding on and off. Chemical degradation of a dendritic scaffold breaks down ligands, temporarily disabling high-affinity interactions with biological targets.

More Related Videos

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
10:17

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

Related Experiment Videos

Last Updated: Jun 23, 2026

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
07:16

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

Published on: February 9, 2024

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
10:17

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

Area of Science:

  • Supramolecular Chemistry
  • Chemical Biology
  • Materials Science

Background:

  • Dendritic scaffolds enable multivalent interactions, crucial for molecular recognition.
  • Controlling these interactions is key for developing dynamic synthetic systems.
  • Existing methods for switching molecular interactions are limited.

Purpose of the Study:

  • To develop a chemical degradation strategy to reversibly control multivalent DNA binding.
  • To demonstrate a method for temporarily endowing molecules with high affinity for biological targets.
  • To explore applications in synthetic systems for intervening in biological pathways.

Main Methods:

  • Synthesis of a dendritic scaffold functionalized with DNA-binding ligands.
  • Induction of chemical degradation of the scaffold using specific reagents.
  • Analysis of DNA binding affinity before and after scaffold degradation using techniques like surface plasmon resonance.

Main Results:

  • Chemical degradation of the dendritic scaffold led to the breakdown of the multivalent ligand array.
  • This breakdown effectively "switched off" the high-affinity interaction with DNA.
  • The process allowed for temporary, tunable control over molecular binding.

Conclusions:

  • Chemical degradation of dendritic scaffolds offers a novel approach to control multivalent interactions.
  • This method provides a mechanism for temporary high-affinity binding to biological targets.
  • The findings have implications for designing responsive synthetic systems for biological intervention.