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Carbon disulfide exposure attenuates adrenergic inotropic response in rats
M Klapperstück1, S Müller, P Hoffmann
1Institute of Industrial Toxicology, Martin-Luther-University, Halle, Saale BRD.
Abstract:
Catecholamine-induced myocardial necrosis is enhanced in carbon disulfide exposed rats. We investigated whether the reported morphological findings after carbon disulfide exposure are accompanied by functional disturbances of the adrenergic inotropic response as well as by biochemical alterations. The cardiac effects of epinephrine and norepinephrine were studied in urethane-anesthetized rats which had been subacutely exposed to carbon disulfide. Compared with not exposed control animals left ventricular inotropic response was diminished and the transient T-wave elevation of the ECG due to ischemic episodes was enhanced in carbon disulfide-exposed rats. Activity of LDH-isozymes in the myocardium was shifted toward LDH-M. Our results support the hypothesis that carbon disulfide causes disturbances of energy supply in the heart.
Insights
Carbon disulfide exposure diminishes the heart's response to catecholamines and alters cardiac energy metabolism in rats. These findings suggest carbon disulfide impairs cardiac function and energy supply.
Area of Science:
- Toxicology
- Cardiovascular Physiology
- Biochemistry
Background:
- Carbon disulfide (CS2) exposure is known to enhance catecholamine-induced myocardial necrosis.
- Morphological changes in the heart following CS2 exposure have been observed.
- The functional and biochemical consequences of these morphological changes require further investigation.
Purpose of the Study:
- To investigate functional disturbances in the adrenergic inotropic response after carbon disulfide exposure.
- To identify biochemical alterations in the myocardium associated with carbon disulfide exposure.
- To determine if carbon disulfide exposure impacts the heart's energy supply mechanisms.
Main Methods:
- Subacute exposure of rats to carbon disulfide.
- Assessment of cardiac effects of epinephrine and norepinephrine in urethane-anesthetized rats.
- Measurement of left ventricular inotropic response and electrocardiogram (ECG) changes.
- Analysis of lactate dehydrogenase (LDH) isozyme activity in myocardial tissue.
Main Results:
- Carbon disulfide-exposed rats exhibited a diminished left ventricular inotropic response to catecholamines compared to controls.
- An enhanced transient T-wave elevation on the ECG, indicative of ischemic episodes, was observed in exposed rats.
- A shift in myocardial LDH-isozyme activity towards LDH-M was detected in carbon disulfide-exposed rats.
Conclusions:
- Carbon disulfide exposure leads to functional impairment of the adrenergic inotropic response in the rat heart.
- Biochemical alterations, including changes in LDH isozyme patterns, suggest disturbances in cardiac energy metabolism.
- The findings support the hypothesis that carbon disulfide negatively impacts cardiac energy supply.