Related Experiment Video
Updated: Jun 23, 2026
An Orthotopic Model of Murine Bladder Cancer
Published on: February 6, 2011
[A simple and efficient method for establishing a mouse model of orthotopic MB49 bladder cancer]
Zhong-kun Liang1, Lin Zhang, Zhi-ming Hu
1Department of Urologic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. liangzhongkun@139.com
Objective:
To establish a simple and efficient method for establishing a mouse model of orthotopic superficial bladder cancer.
Methods:
C57BL/6 mice were anesthetized with sodium pentobarbital and catheterized with modified IV catheter (24 G). The mice were intravesically pretreated with HCl and then with NaOH, and after washing the bladders with phosphate-buffered saline (PBS), 100 microl (1 x 10(7)) MB49 cells were infused and allowed to incubate in the bladder for 2 h followed intravesical mitomycin C (MMC) administration. The tumor formation rate, survival, gross hematuria, and bladder weight were determined as the outcome variables, and the pathology of the bladders was observed.
Results:
Instillation of MB49 tumor cells resulted in a tumor formation rates of 100% in all the pretreated groups while 0% in the control group without pretreatment. MMC significantly reduced the bladder weight as compared to PBS.
Conclusion:
We have successfully established a stable, reproducible, and reliable orthotopic bladder cancer model in mice.
