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Updated: Jun 23, 2026
09:11
In Vitro Apical-Out Enteroid Model of Necrotizing Enterocolitis
Published on: June 8, 2022
[Necrotic cells induce and promote inflammatory responses in vitro]
Xuan Liu1, Yu-wei Luo, Er-wei Sun
1Institute of Transplant Immunology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. xuan2225@fimmu.com
Summary
Necrotic cells significantly increase the secretion of inflammatory cytokines like TNF-alpha and IL-6 by macrophages. These cells also enhance the inflammatory response when combined with lipopolysaccharide (LPS).
Area of Science:
- Immunology
- Cell Biology
Background:
- Inflammation is a critical biological response.
- The role of necrotic cells in modulating immune responses requires further elucidation.
Purpose of the Study:
- To investigate the impact of necrotic cells on inflammatory cytokine secretion by macrophages.
Main Methods:
- RAW264.7 macrophages were co-cultured with heat-induced necrotic mouse thymocytes.
- Cytokine levels (TNF-alpha, IL-6) were measured using a Bio-Plex suspension system, with and without lipopolysaccharide (LPS).
Main Results:
- Co-culture with necrotic cells significantly enhanced TNF-alpha and IL-6 secretion by macrophages.
- Necrotic cells synergistically increased LPS-induced pro-inflammatory cytokine production.
Conclusions:
- Necrotic cells independently induce pro-inflammatory cytokine expression.
- Necrotic cells play a significant role in initiating and sustaining inflammatory responses, particularly in conjunction with stimuli like LPS.
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01:38Inflammation
Overview
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