[Effect of angiotensin1-7 on alpha-smooth muscle actin protein expression in rat hepatic stellate cells]
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Updated: Jun 23, 2026
Seven Steps to Stellate Cells
Published on: May 10, 2011
Song-song Ying1, Xu Li, Mao-liang Huang
1Department of Digestive Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Objective:
To investigate the effect of angiotensin II and angiotensin1-7 on alpha-smooth muscle actin (alpha-SMA)-induced Ca(2+)-independent pathways mediated by Rho kinase2 in hepatic stellate cells (HSCs).
Methods:
HSC-T6 cells were treated with 10 micromol/L of AngII, Ang1-7, AngII +Ang1-7, and Ang1-7+A779. RT-PCR was used to detect the expression of Rho kinase2 (Rock2) in Ca(2+)-independent pathways, and alpha-SMA protein expression was detected by Western blotting.
Results:
The mRNA expression of Rock2 increased significantly in the cells after AngII treatment (P<0.01), but decreased following Ang1-7 treatment. Ang1-7 treatment significantly reduced alpha-SMA level in AngII-induced cells (P<0.01).
Conclusion:
Ang1-7 can inhibit AngII-induced activation of Rock2 and reduce alpha-SMA expression in HSCs.
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